sa 11  (ATCC)

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Drug repurposing of Nifuratel against methicillin-resistant Staphylococcus aureus through proton motive force disruption

doi: 10.3389/fcimb.2025.1738031

Figure Lengend Snippet: Antibiofilm and anti-virulence activity by Nifuratel. (A, B) Biofilm inhibitory activity against ATCC 43300 determination by crystal violet staining (A) and CFU counting assay (B) , respectively. Dotted line indicates limit of detection. (C, D) Pre-formed biofilm eradicating activity against ATCC 43300 determination by crystal violet staining (C) and CFU counting assay (D) , respectively. Dotted line indicates limit of detection. (E, F) Biofilm inhibition (E) and eradication (F) activities detection by SYTO9/PI staining. The concentrations of Nifuratel used for the biofilm inhibitory and eradicating assays were 4 and 8 μg/mL, respectively. (G) Hemolytic activity of MRSA on the sheep blood agars in the presence or absence of 4 μg Nifuratel. SA-11 was selected for this assay due to its strong hemolytic activity. (H) Hemolytic activity quantitative of Nifuratel at the concentration of 1/2× MIC. (I) Surface spreading inhibition by sub-MICs of Nifuratel. (J) Auto-aggregation of ATCC 43300 in the presence or absence of 1/2× MIC Nifuratel. * P < 0.05. ** P < 0.01. *** P < 0.001. **** P < 0.0001.

Article Snippet: The low resistance development probability by Nifuratel was also observed by ATCC 25923 and SA-11 ( ).

Techniques: Activity Assay, Staining, Inhibition, Concentration Assay

Journal: bioRxiv

Article Title: Microbiota-derived indole metabolites inhibit rotavirus infection in vitro and in vivo and in human infants

doi: 10.1101/2025.10.29.685378

Figure Lengend Snippet: (a) Schematic overview of oral rotavirus vaccine (ORV) challenge study in healthy adults. (b) Volcano plot of differentially abundant fecal metabolites between control and all antibiotic-treated groups (log2foldchange >1 or <-1; p.adj <0.05, Mann-Whitney U test with FDR correction). Metabolites belonging to the tryptophan group are labeled. (c) Relative abundance of select tryptophan metabolites in RV shedders (n=20) and non-shedders (n=43). The bottom header of each panel shows the metabolite names, top header indicates the pathway classification (kynurenine or indole pathway). Boxplot indicates first, second and third quartiles of data, whiskers indicate maximum and minimum values. P-values are based on Wilcoxon test with FDR correction (ns: non-significant, * p< 0.05; ** p< 0.01; *** p< 0.001).

Article Snippet: Rotavirus WA strain was activated by adding 10 μg/ml trypsin (Worthington biochemical, #9002-07-7) followed by incubation at 37°C for 1 hour.

Techniques: Control, MANN-WHITNEY, Labeling

Journal: eBioMedicine

Article Title: Loss of function of Adducin 3 (ADD3) causes abnormal development and impaired barrier function of human and mouse bile duct cells resulting in increased incidence and severity of Biliary Atresia

doi: 10.1016/j.ebiom.2025.106052

Figure Lengend Snippet: Add3 +/− and Add3−/− mouse exhibited a more severe RRV-induced BA phenotypes. (A) Representative pictures of the morphology (Light; Green Fluorescence), histology (H&E) and Sirius Red staining of livers of wild-type ( Add3+/+ ) non-BA neonates (n = 8); RRV-infected wild-type ( Add3+/+ ; n = 16); Add3+/− (n = 17) and Add3−/− (n = 14) BA neonates (Experimental BA) at post-inoculation day 28. “∗” indicates necrotic areas, and was magnified as shown as insets. Arrows indicate portal and periportal fibrosis. Arrowheads indicate portal-to-portal (P-P) bridging fibrosis. (B) Body weight (Mean ± SD) of wild-type ( Add3+/+ ; n = 16); Add3+/− (n = 17) and Add3−/− (n = 14) BA neonates at post-inoculation day 28. Comparative analysis between groups was performed using Kruskal–Wallis test, and p values were shown. Percentages of liver necrosis (C) and fibrosis (D) in wild-type ( Add3+/+ ; n = 16); Add3+/− (n = 17) and Add3−/− (n = 14) BA neonates were shown as Mean ± SD, and p values were shown (Kruskal–Wallis test for quantitative analysis between groups, Dunn's test for multiple comparisons between groups). (E) Serum total bilirubin (TBil), alkaline phosphatase (ALP) and total bile acids levels of wild-type (WT), Add3+/− (C) and Add3−/− (D) neonates with (BA+) and without BA (BA-) phenotypes. Data (Mean ± SD) was analysed between groups using Kruskal–Wallis test, multiple comparisons between groups were performed using Dunn's test, and p values were shown. (WT: BA+, n = 4; BA-, n = 6; Add3+/− : BA+, n = 8; BA-, n = 7; Add3−/− : BA+, n = 4; BA-, n = 5). Abbreviations: li, liver; gb, gall bladder.

Article Snippet: Postnatal day 2 mouse neonates from crossing of Add3+/− mice were injected with RRV (rhesus rotavirus; 20 μl of 1 × 10 6 pfu/ml RRV, MMU 18006, ATCC® VR-1739TM) via peritoneal route.

Techniques: Fluorescence, Staining, Infection

Journal: bioRxiv

Article Title: Microbiota-derived indole metabolites inhibit rotavirus infection in vitro and in vivo and in human infants

doi: 10.1101/2025.10.29.685378

Figure Lengend Snippet: (a) Schematic overview of oral rotavirus vaccine (ORV) challenge study in healthy adults. (b) Volcano plot of differentially abundant fecal metabolites between control and all antibiotic-treated groups (log2foldchange >1 or <-1; p.adj <0.05, Mann-Whitney U test with FDR correction). Metabolites belonging to the tryptophan group are labeled. (c) Relative abundance of select tryptophan metabolites in RV shedders (n=20) and non-shedders (n=43). The bottom header of each panel shows the metabolite names, top header indicates the pathway classification (kynurenine or indole pathway). Boxplot indicates first, second and third quartiles of data, whiskers indicate maximum and minimum values. P-values are based on Wilcoxon test with FDR correction (ns: non-significant, * p< 0.05; ** p< 0.01; *** p< 0.001).

Article Snippet: The human rotavirus WA strain (G1P8) was purchased from American Type Culture Collection (ATCC, Rotavirus A-VR-2018) and propagated on MA104 cells with no FBS supplementation.

Techniques: Control, MANN-WHITNEY, Labeling